Synthesis and anti-staphylococcal activity of TiO2 nanoparticles and nanowires in ex vivo porcine skin model.

Synthesis and anti-staphylococcal activity of TiO2 nanoparticles and nanowires in ex vivo porcine skin model.

Journal of biomedical nanotechnology (2014-04-17)

Namrata Nataraj, G S Anjusree, Asha Anish Madhavan, P Priyanka, Deepthi Sankar, N Nisha, S V Lakshmi, R Jayakumar, Avinash Balakrishnan, Raja Biswas

PMID24734539

ABSTRACT

Staphylococcus aureus is one of the major causes of skin and soft tissue infections. In this study we compared the antimicrobial activity of two different TiO2 nanoformulations against Staphylococcus aureus. We synthesized TiO2 nanoparticles of approximately 80 nm diameter and TiO2 nanowires of approximately 100 nm diameter. Both nanoformulations possess anti-microbial activity; were non-hemolytic and cytocompatible. However, the anti-staphylococcal activity of TiO2 nanowires was better than the nanoparticles. In broth culture, growth of S. aureus was only partially inhibited by 2% and 4 wt% TiO2 nanoparticles and completely inhibited by TiO2 nanowires till 24 h. TiO2 nanowires treated S. aureus cells exhibits diminished membrane potential than nanoparticle treated cells. The anti-microbial properties of both TiO2 nanoformulations were validated using ex vivo porcine skin model which supplements the in vitro assays. Anti-bacterial activity of the TiO2 nanowires were also validated against multi drug resistant pathogenic strains of S. aureus, showing the clinical potency of the TiO2 nanowires compared to its nanoparticles.

MATERIALS

Product Number

Brand

Product Description

GF52005596

Titanium, tube, 500mm, outside diameter 9.5mm, inside diameter 8.2mm, wall thickness 0.65mm, annealed, 99.6+%

GF51463357

Titanium, tube, 200mm, outside diameter 10.3mm, inside diameter 8.7mm, wall thickness 0.8mm, annealed, 99.6+%

GF58567067

Titanium, rod, 100mm, diameter 5mm, annealed, 99.6+%

GF54883581

Titanium, rod, 1000mm, diameter 3.0mm, annealed, 99.6+%

GF56868766

Titanium, wire reel, 25m, diameter 1.0mm, annealed, 99.6+%

GF41437830

Titanium, tube, 100mm, outside diameter 1.05mm, inside diameter 0.75mm, wall thickness 0.15mm, annealed, 99.6+%

GF91859967

Titanium, rod, 100mm, diameter 9.5mm, annealed, 99.99+%

GF98611623

Titanium, rod, 200mm, diameter 8.0mm, as drawn, 99.99+%

GF71783354

Titanium, wire reel, 100m, diameter 0.75mm, as drawn, 99.6+%

GF13900205

Titanium, tube, 100mm, outside diameter 0.51mm, inside diameter 0.35mm, wall thickness 0.08mm, annealed, 99.6+%

GF26602321

Titanium, tube, 500mm, outside diameter 25.4mm, inside diameter 23.62mm, wall thickness 0.89mm, annealed, 99.6+%

GF72193114

Titanium, tube, 500mm, outside diameter 2.03mm, inside diameter 1.63mm, wall thickness 0.2mm, annealed, 99.6+%

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Titanium, rod, 500mm, diameter 6mm, annealed, 99.6+%

GF17705516

Titanium, rod, 200mm, diameter 3.0mm, annealed, 99.6+%

GF46132534

Titanium, wire reel, 100m, diameter 0.25mm, as drawn, 99.6+%

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Titanium, tube, 200mm, outside diameter 6.35mm, inside diameter 5.53mm, wall thickness 0.41mm, annealed, 99.6+%

GF65336700

Titanium, rod, 200mm, diameter 2mm, annealed, 99.6+%

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Titanium, tube, 1000mm, outside diameter 1.6mm, inside diameter 1.2mm, wall thickness 0.2mm, hard, 99.6+%

GF14377631

Titanium, wire reel, 25m, diameter 0.25mm, annealed, 99.6+%

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Titanium, wire reel, 500m, diameter 0.20mm, annealed, 99.6+%

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Titanium, wire reel, 25m, diameter 0.25mm, as drawn, 99.6+%

GF95731960

Titanium, tube, 500mm, outside diameter 3.0mm, inside diameter 1.5mm, wall thickness 0.75mm, annealed, 99.6+%

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Titanium, rod, 100mm, diameter 2mm, annealed, 99.6+%

GF94150593

Titanium, wire reel, 10m, diameter 1.5mm, as drawn, 99.6+%

GF96391402

Titanium, wire reel, 25m, diameter 0.20mm, annealed, 99.6+%

GF76266423

Titanium, wire reel, 0.5m, diameter 1.0mm, annealed, 99.6+%

266035

Sigma-Aldrich

Titanium, wire, diam. 1.0 mm, 99.99% trace metals basis

635057

Sigma-Aldrich

Titanium(IV) oxide, rutile, <001>, (single crystal substrate), ≥99.9% trace metals basis, L × W × thickness 10 mm × 10 mm × 0.5 mm

460400

Sigma-Aldrich

Titanium, wire, diam. 0.25 mm, 99.7% trace metals basis

369489

Sigma-Aldrich

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