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Merck

[Modification of the method of preparation of paraffin blocks].

Arkhiv patologii (1984-01-01)
V G Shlopov, N A Krivenko, T I Shevchenko
ABSTRACT

The quality of paraffin sections is determined to a considerable degree by the properties of paraffin block such as crystal size and spatial homogeneity. A new temperature regimen of treatment in preparation of paraffin blocks has been developed. After impregnation, pieces of tissue are overlayed with paraffin at a temperature of 56-60 degrees C in paper-tin foil dishes and then immediately placed in an antifreeze at 0 degree to -5 degrees C. The internal stresses, deformations, and microcracks occurring in this procedure are eliminated by incubation of the resulting block in a thermostat at 45 degrees C in vapours of low-melting fraction of paraffin (2-24 hours) followed by rapid cooling with cold air (from -5 degrees to -10 degrees C). The second step of the treatment may also be used for improvement of the quality of paraffin blocks after long-term storage.

MATERIALS
Product Number
Brand
Product Description

Tin, foil, 25x25mm, thickness 0.20mm, as rolled, 98.8%
Tin, foil, 300x300mm, thickness 0.1mm, as rolled, 99.95%
Tin, foil, 25x25mm, thickness 0.75mm, as rolled, 99.99+%
Tin, foil, 10m coil, thickness 0.007mm, 97.4%
Tin, foil, 0.5m coil, thickness 0.0125mm, 97.4%
Tin, foil, 10m coil, thickness 0.109mm, coil width 41mm, as rolled, 99.95%
Tin, foil, 100x100mm, thickness 1.0mm, as rolled, 98.8%
Tin, foil, 0.5m coil, thickness 0.125mm, as rolled, 98.8%
Tin, foil, 300x300mm, thickness 0.5mm, as rolled, 98.8%
Tin, foil, 10m coil, thickness 0.084mm, coil width 41mm, as rolled, 99.95%
Tin, foil, 50x50mm, thickness 0.15mm, as rolled, 98.8%
Tin, foil, 150x150mm, thickness 0.5mm, as rolled, 99.99+%
Tin, foil, 150x150mm, thickness 1.5mm, as rolled, 99.99+%
Tin, foil, 50x50mm, thickness 0.75mm, as rolled, 99.99+%
Tin, foil, 150x150mm, thickness 2.0mm, as rolled, 99.99+%
Tin, foil, 0.2m coil, thickness 0.05mm, as rolled, 99.99+%
Tin, foil, 300x300mm, thickness 0.15mm, as rolled, 98.8%
Tin, foil, 10m coil, thickness 0.0125mm, 97.4%
Tin, foil, 25x25mm, thickness 0.125mm, as rolled, 98.8%
Tin, foil, 100x100mm, thickness 1.0mm, as rolled, 99.999%
Tin, foil, 15mm disks, thickness 0.009mm, 97.4%
Tin, foil, 300x300mm, thickness 2.0mm, as rolled, 98.8%
Tin, foil, 25x25mm, thickness 1.0mm, as rolled, 99.999%
Tin, foil, 25x25mm, thickness 0.5mm, as rolled, 99.99+%
Tin, foil, 100x100mm, thickness 2.0mm, as rolled, 99.99+%
Tin, foil, 15mm disks, thickness 0.02mm, 99.75%
Tin, foil, 300x300mm, thickness 0.125mm, as rolled, 98.8%
Tin, foil, 10mm disks, thickness 0.05mm, as rolled, 99.99+%
Tin, foil, 100x100mm, thickness 0.65mm, as rolled, 99.99+%
Tin, foil, 300x300mm, thickness 0.20mm, as rolled, 98.8%