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Key Documents

MSKT0002

Sigma-Aldrich

SOLu-Trypsin Rapid Digestion Kit

Proteomics grade

Synonym(s):

Rapid Trypsin, Recombinant Trypsin for Protein Digestion in Mass Spectrometry, rTrypsin

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About This Item

UNSPSC Code:
41105331
NACRES:
NA.78

grade

Proteomics Grade

form

solution

suitability

suitable for mass spectrometry

shipped in

wet ice

storage temp.

2-8°C

General description

SOLu-Trypsin Rapid Digestion Kit (MSKT0002) consists of 400 ug of solution-stable trypsin (EMS0004) suitable for protein sequencing, and 30 mL of Rapid Digestion Buffer (EMS0009), allowing for trypsin digestion in one hour at 60°C. Trypsin should be used at a 1:10 enzyme:substrate ratio in Rapid Digestion Buffer, which can be substituted into existing user workflows.

Features and Benefits

  • Provides a fast and efficient method for protein digestion.
  • Removal of denaturants by offline desalting is not necessary, which further shortens the time for sample preparation.
  • Compatible with a variety of sample types, reduction and alkylation protocols, and downstream workflows.

Storage Class Code

11 - Combustible Solids


Certificates of Analysis (COA)

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Articles

Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation.

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