I5381

IgG from mouse serum

reagent grade, ≥95% (SDS-PAGE), lyophilized powder

• Synonym(s): Mouse IgG
• MDL number: MFCD00212351
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• conjugate: unconjugated
• grade: reagent grade
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• composition: Protein, ≥80%
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Mouse IgG is purified from normal mouse serum by fractionation and ion-exchange chromatography.

Application

Purified mouse IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Mouse IgG was used as standard in ELISA and chromatin immunoprecipitation assay. It was used to coat nano particles in the development of biosensors.

Physical form

Lyophilized from 0.01 M phosphate buffer, pH 7.2, with 0.015 M NaCl

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves