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H0413

Millipore

HIS-Select® Filter Plate

Synonym(s):

His-tag filtered microplate

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About This Item

UNSPSC Code:
41121800
NACRES:
NB.24

grade

for molecular biology

form

plate

storage temp.

2-8°C

General description

The HIS-Select® Filter Plate is an immobilized metal-ion affinity chromatography (IMAC) product that allows rapid purification of small-scale crude cell extracts containing histidine-tagged proteins. The HIS-Select® Filter Plate contains 20 μm, spherical silica particles (100 nm pore size) with a hydrophilic layer.

The HIS-Select® Filter Plate is selective for recombinant proteins with histidine tags and exhibits very low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography. The binding capacity of the HIS-Select® Filter Plate is typically > 2 mg per well as determined with an approximately 30 kDa histidine-tagged protein.

Application

The HIS-Select® Filter Plate is suitable for purification of recombinant proteins with histidine tags.

Features and Benefits

  • High Selectivity for his-tagged proteins
  • Ready-to-use plate format
  • Compatible with CelLytic™ cell lysis reagents

Legal Information

Covered by US Patent No 6,623,655 and are sold for research use only. Commercial use requires additional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 3 - Carc. 1A Inhalation - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation

Target Organs

Respiratory Tract

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Benjamin D Allen et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(46), 19838-19843 (2010-11-04)
The stability, activity, and solubility of a protein sequence are determined by a delicate balance of molecular interactions in a variety of conformational states. Even so, most computational protein design methods model sequences in the context of a single native
Isabel K Macdonald et al.
PloS one, 7(7), e40759-e40759 (2012-07-21)
An assay employing a panel of tumor-associated antigens has been validated and is available commercially (EarlyCDT®-Lung) to aid the early detection of lung cancer by measurement of serum autoantibodies. The high throughput (HTP) strategy described herein was pursued to identify

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