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Key Documents

03-0155-00

Millipore

SMC® Human Interleukin 6 (IL-6) High Sensitivity Immunoassay Kit

for quantification of IL-6 in human serum and plasma.

Synonym(s):

B-cell stimulatory factor 2 immunoassay kit, IL 6 immunoassay kit, IL-6 Single Molecule Counting Kit, IL-6 ultra-sensitive immunoassay

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

species reactivity

human

manufacturer/tradename

SMC®

assay range

sensitivity: 0.08 pg/mL
(lower limit of quantification)

inter-assay cv: <20%
intra-assay cv: <15%
(Intra-assay CV n=20; Inter-assay n=6)

technique(s)

ELISA: suitable
single molecule counting: suitable

General description

Interleukin 6 (IL-6) is secreted by T cells and macrophages and acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Some of its functions include the stimulation of the immune response during infection, induction of fever in autoimmune, infectious and non-infectious diseases, the stimulation of acute phase reactions, differentiation of T cells, proliferation and differentiation of B-cells, the development of blood cells as well as the activation of osteoclasts and osteoporosis and the secretion of vascular endothelium growth factor (VEFG) to increase blood vessel growth and vascular permeability in inflammation. It is important for a short-term defense against infection, injury and inflammation however dysregulation of IL-6 results in disease.

IL-6 is important in the study of diseases such as diabetes, lupus, multiple myeloma, rheumatoid arthritis and some cancers such as prostate and pancreatic cancer. Conversely, deficiency of IL-6 can have an effect on immune activation and IgA antibodies while overexpression of IL-6 can result in an immunological imbalance leading to an autoimmune pathology.

Specificity

Although IL-6 was detectable in non-human primate (rhesus macaque and cynomolgus monkey) samples, the assay has not been validated for these species.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Inflammatory Disease

Immune Response

Autoimmune disease
The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC Human Interleukin 6 (IL-6) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-6 in human serum and plasma.
This new streamline kit has been improved with the following features while the kit performance is consistent with the legacy IL-6 kit, #03-0089-01:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Overnight incubation can be used
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO® and Erenna

Components

Assay Buffer

Human IL-6 Beads

Standard Diluent

Human IL-6 Detection Antibody

Human IL-6 Standard

10X Wash Buffer

Buffer D

Elution Buffer B

Specifications

Minimum of 5 samples spiked with average recovery of 107% with a range of 90-114%.

Legal Information

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificates of Analysis (COA)

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Carolyne Pimentel Rosado et al.
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 46(12), 1469-1475 (2021-07-01)
This study evaluated the effect of green banana flour (GBF) consumption on obesity-related conditions in mice fed high-fat diets. GBF was prepared using stage 1 green banana pulp, which was dehydrated and milled. Mice were fed a control diet (n =

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