C2993

Anti-CRMP2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Collapsin Response Mediator Protein 2, Anti-Dihydropyriminidase Related Protein-2 (DRP2, DRP-2, DHPRP2), Anti-Dihydropyriminidase-like 2 (DPYSL2), Anti-TOAD-64
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03456049
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 34

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~62 kDa
• species reactivity: human, mouse, rat
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): western blot: 0.1-0.2 μg/mL using HeLa whole cell lysate and mouse brain extract (S1 fraction)
• UniProt accession no.: Q16555
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DPYSL2(1808) ; mouse ... Dpysl2(12934) ; rat ... Dpysl2(25416)

Description

General description

Collapsin response mediator proteins CRMPs) consist of a family of cytosolic phosphoproteins expressed in the nervous system and involved in neuronal differentiation and axonal guidance.

Application

Anti-CRMP2 antibody produced in rabbit has been used in:

• western blotting
• immunohistofluorescence
• epifluorescence imaging
• coimmunoprecipitation[1]

Biochem/physiol Actions

CRMPs are a part of the collapsin/semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. CRMP2 is upregulated during development, and appears to be crucial for axon outgrowth. Glycogen synthase kinase 3 beta (GSK-3b) phosphorylates and inactivates CRMP-2 downstream of the phosphoinositide-3-kinase (PI3K/Akt) pathway, thus regulating neuronal polarity. CRMP2 interacts with tubulin dimers, kinesin-1 and WASP-family verprolin homologous protein 1 (WAVE1) complex to regulate axon outgrowth.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable