Skip to Content
Merck

Skip To

A9046

Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti Chicken Igy

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p)
Citations:
95
Pricing and availability is not currently available.
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


biological source

rabbit

Quality Segment

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

chicken

technique(s)

direct ELISA: 1:30,000, dot blot: 1:160,000 (indirect chemiluminescence), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgG.

Immunogen

purified chicken IgG

Application

Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody produced in rabbit has been used in indirect enzyme-linked immunosorbent assay (ELISA), dierct ELISA, dot blot and immunohistochemistry.
Proteins isolated from rabbit glomeruli by iron oxide magnetization were subjected to western blot analysis using HRP-conjugated rabbit anti-chicken IgY as the secondary antibody.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for immunoprecipitation and western blot (1:2,000) assays. The antibody can also be used for direct ELISA (1:30,000), dot blot (1:12,000-1:15,000) and IHC (1:1,000) applications.

Biochem/physiol Actions

Egg yolk IgY immunoglobulin acquired from hen have been studied extensively. It is useful in immunological assays and does interact with the rheumatoid factor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
A5670A8667A9792
species reactivity

chicken

species reactivity

pig

species reactivity

human

species reactivity

chicken

biological source

rabbit

biological source

rabbit

biological source

goat

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000, dot blot: 1:160,000 (indirect chemiluminescence)

technique(s)

direct ELISA: 1:40,000, dot blot: 1:150,000 (chemiluminescent)

technique(s)

direct ELISA: 1:30,000, dot blot: 1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

technique(s)

direct ELISA: 1:30,000, dot blot: 1:160,000 using indirect chemiluminescence system using 5 ng human IgG/dot and chicken anti-human IgG as the primary antibody and Luminol plus enhancer as the substrate., immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using using human pancreas sections and chicken anti-human insulin as the primary antibody.

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice


Still not finding the right product?

or

Try our Product Selector Tool to narrow your options


pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library


Related Content

Instructions






Global Trade Item Number

SKUGTIN
A9046-1ML04061838443663

Questions

1–5 of 5 Questions  
  1. Do we have additional anti-chicken IgY conjugates?

    1 answer
    1. We provide anti-chicken IgY antibodies that are conjugated to alkaline phosphatase (Product No. A9171) and FITC (Product No. F8888).  We also provide the unconjugated anti-chicken IgY antibody (Product No. C2288).

      Helpful?

  2. Product A9046, Anti-Chicken IgY, is peroxidase conjugated.  Is this similar to horseradish peroxidase?

    1 answer
    1. The peroxidase used to make this product is indeed peroxidase from horseradish (HRP).

      Helpful?

  3. How is chicken IgY different from chicken IgG?

    1 answer
    1. Chicken IgG refers to antibodies from the chicken serum and chicken IgY antibodies are from the yolk.

      Helpful?

  4. Will Product A9046, Anti-Chicken IgY, also recognize chicken IgG?

    1 answer
    1. This antibody (and possibly other anti-chicken antibodies) recognizes both chicken IgG from serum and chicken IgY from egg yolk, because these immunoglobulins are structurally very similar.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

Reviews

No rating value

Active Filters