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Lipase from Rhizopus oryzae

Name: Lipase from <I>Rhizopus oryzae</I>
Brand: SIGMA

powder (fine), ~10 U/mg

Synonym(s):

Lipase from Rhizopus arrhizus, Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

CAS Number:

9001-62-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-619-9

EC Number:

3.1.1.3 (BRENDA, IUBMB)

MDL number:

MFCD00131509

Specific activity:

~10 U/mg

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Properties

form

powder (fine)

Quality Segment

100

specific activity

~10 U/mg

mol wt

M_r ~43000

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

Description

General description

Research Area: Cell Signaling
Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a presequence of 26 amino acids, followed by a prosequence of 97 amino acids, which is attached to the N-terminal of a mature sequence consisting of 269 amino acids.

Lipase from Rhizopus oryzae (ROL) comprises an oxyanion hole, four N-glycosylation sites, and an active site region. It possesses N-terminal presequence and prosequence.

Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Lipase from Rhizopus oryzae has been used:

to test its effect on 1,2-diolein synthesis and triolein ethanolysis
for immobilization on graphene oxide support for biocatalysis studies
to digest triglycerides (TAG) from Chlamydomonas reinhardtii and S. cerevisiae[1]

Biochem/physiol Actions

Rhizopus oryzae lipase (ROL) has been extensively researched for its regiospecificity in biodiesel production. Due to its remarkable characteristics, including 1,3-specificity, high enantioselectivity, and stability in organic solvents, ROL has garnered significant attention for applications in the energy, food, and pharmaceutical industries.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Lipase from Rhizopus oryzae (ROL) acts as a catalyst for the enzymatic biosynthesis of polyglycerol polyricinoleate through a reversal of hydrolysis.[2] ROL is useful in the industrial production of structured lipids due to its 1,3-regiospecificity functionality.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol of butyric acid per minute at pH 8.0 and 40°C (tributyrin, Cat. No. 91010 as substrate) 5000 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314 as substrate, at pH 8.0 and 40°C

Catalyst for the interesterification of oils and fats; For removal of interfering triglycerides in the electroimmunoassay of apolipoprotein B; Racemic epoxy ester resolution through enantioselective enzymatic hydrolysis.

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

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This Item	62310	62316	62285
Sigma-Aldrich 62305 Lipase from Rhizopus oryzae	Sigma-Aldrich 62310 Lipase from Rhizopus niveus	Sigma-Aldrich 62316 Lipase from Candida rugosa	Sigma-Aldrich 62285 Lipase from Aspergillus oryzae
specific activity ~10 U/mg	specific activity ≥1.5 U/mg	specific activity ≥2 U/mg	specific activity ~50 U/mg
form powder (fine)	form powder (fine)	form powder	form powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
mol wt M_r ~43000	mol wt M_r ~83000	mol wt -	mol wt -
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100