ROAHAHA

Anti-HA High Affinity

from rat IgG₁

• Synonym(s): antibody
• UNSPSC Code: 12352203
• NACRES: NA.54
• Conjugate: unconjugated
• Clone: 3F10, monoclonal
• Application: —
• Citations: 41

Properties

• biological source: rat
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 3F10, monoclonal
• form: lyophilized
• packaging: pkg of 50 μg (11867423001), pkg of 500 μg (11867431001)
• manufacturer/tradename: Roche
• isotype: IgG1
• epitope sequence: YPYDVPDYA
• storage temp.: 2-8°C

Description

General description

Anti-HA High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10). Anti-HA High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".

Immunogen

Amino acids 98-106 from the human influenza virus hemagglutinin protein

Application

Use Anti-HA High Affinity for the detection of native influenza hemagglutinin protein and recombinant proteins that contain the HA epitope using:

• Dot blots
• ELISA
• Immunocytochemistry
• Immunoprecipitation[1]
• Western blots[2][3]

Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.

Biochem/physiol Actions

Anti-HA, High Affinity recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:

• ELISA: for detection 100 ng/ml; for coating 1 to 5 μg/ml
• Immunoprecipitation: 0.5 to 5 μg/ml
• Western blot: 50 to 200 ng/ml

Add 0.5 ml (11 867 423 001; 50 μg) or 2.5 ml (11 867 431 001; 500 μg) double-distilled water to a final concentration of 100 μg/ml or 200 μg/ml respectively.
Rehydrate for 10 min prior to use.

Analysis Note

Function tested in western blot.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictograms: GHS07,GHS07
• signalword: Warning-Warning
• hcodes: H317 - H412 - H317
• pcodes: P261 - P273 - P280 - P261 - P272 - P280 - P333 + P313 - P362 + P364 - P333 + P313 - P362 + P364 - P501 - P501
• Hazard Classifications: Aquatic Chronic 3 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: does not flash
• flash_point_c: does not flash