HomeSolid Phase Microextraction (SPME)GC/MS/MS Analysis of Bisphenol A (BPA) in a Fruit Flavored Energy Drink on the SLB-PAHms Column After SPME Using an Overcoated SPME Fiber
GC/MS/MS Analysis of Bisphenol A (BPA) in a Fruit Flavored Energy Drink on the SLB-PAHms Column After SPME Using an Overcoated SPME Fiber
Figure 1. GC/MS/MS Analysis of Bisphenol A
Conditions
Sample Prep Method | |
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SPME fiber | overcoated PDMS/DVB, 23 gauge |
Extraction | immersion, 50 min, 50 °C, 250 rpm, vial penetration 34 mm |
Desorption process | 3 min, 260°C |
Other sample prep information | Sample was incubated prior to extraction for 10 min at 50°C, 400 rpm mixing. Fiber was washed post-extraction and prior to desorption in water for 0.5 min. After desorption, fiber was post-baked at 270°C for 6 min. |
Primary Analytical Method | |
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Instrument | Agilent® 7890/7000C GC/MS/MS |
Column | SLB-PAHms, 30 m x 0.25 mm I.D., 0.25 µm |
Oven | 100 °C (3 min), 15 °C/min to 300 °C (10 min) |
Inj. temp. | 260 °C |
Carrier gas | helium, 1 mL/min constant flow |
Detector | MS/MS |
MSD interface | 325 °C |
Scan range | MRM: BPA: 213/119, 213/91, 119/91 BPA-d16: 224/215, 224/97, 125/97 |
Injection | Splitless |
Liner | 0.75 mm ID straight (for SPME) |
Description | |
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Analysis Note | Use of bisphenol A (BPA)-based plastics in food and beverage containers continues to be a point of controversy. This study demonstrates the solid phase micro extraction (SPME) of BPA from a fruit flavored energy drink using an overcoated PDMS/DVB fiber (overcoated with PDMS), followed by GC/MS/MS analysis using SLB-PAHms GC Column. At a 10 ppb spiking level, accuracy of the SPME method was 115% with an RSD of 1% for spiked replicates. |
Featured Industry | Food & Beverage |
Legal Information | Agilent is a trademark of Agilent Technologies, Inc. Thermogreen is a trademark of Sigma-Aldrich Co. LLC Redi-Dri is a trademark of Sigma-Aldrich Co. LLC SLB is a trademark of Sigma-Aldrich Co. LLC |
Application No. | CLA010218A |
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