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  • Surface modification of electrospun polyvinylidene fluoride nanofiber membrane by plasma treatment for protein detection.

Surface modification of electrospun polyvinylidene fluoride nanofiber membrane by plasma treatment for protein detection.

Journal of nanoscience and nanotechnology (2013-05-08)
Eugene Cho, Chan Kim, Ju-Young Park, Cheol Ho Hwang, Jin Hee Kim, Yoong Ahm Kim, Morinobu Endo, Duck Rye Chang, Joong-Ki Kook
ABSTRACT

Electrospun polyvinylidene fluoride (PVDF) nanofiber membranes have 3-dementional (3-D) open pore channel and hence have excellent application potential in Western blot. In this study we have modified electrospun PVDF nanofiber membrane by argon (Ar) plasma treatment to improve the surface hydrophilic and detection sensitivity. The results showed that the detection sensitivity of the Ar plasma-treated PVDF nanofiber membrane increased with increasing plasma treatment time without the need for a methanol pre-wet step. This suggests that the Ar plasma treated PVDF nanofiber membrane can be useful in Western blot with high sensitivity and without methanol pre-wet step.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poly(vinylidene fluoride), average Mw ~180,000 by GPC, average Mn ~71,000, beads or pellets
Sigma-Aldrich
Poly(vinylidene fluoride), average Mw ~275,000 by GPC, average Mn ~107,000, pellets
Sigma-Aldrich
Poly(vinylidene fluoride), average Mw ~530,000, pellets
Sigma-Aldrich
Poly(vinylidene fluoride), average Mw ~534,000 by GPC, powder