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T6319

Sigma-Aldrich

Anti-TOM22 antibody , Mouse monoclonal

clone 1C9-2, purified from hybridoma cell culture

Synonym(s):

Anti-Mitochondrial import receptor Tom22, Anti-TOMM22, Anti-Translocase of outer mitochondrial membrane 22 homolog (yeast)

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About This Item

MDL number:
MFCD03457578
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

1C9-2, monoclonal

form

buffered aqueous solution

mol wt

antigen 22 kDa

species reactivity

human, monkey

should not react with

rodent

concentration

~1 mg/mL

technique(s)

immunocytochemistry: suitable
microarray: suitable
western blot: 0.5-1.0 μg/mL using whole extract of cultured human lymphoma Raji cells.

isotype

IgG1

UniProt accession no.

Q9NS69

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TOMM22(56993)

General description

Translocase of outer mitochondrial membrane 22 (TOMM22) is an essential receptor of the mitochondrial import channel. It is characterized with an N-terminal negatively charged region exposed to the cytosol, a putative transmembrane region and a C-terminal intermembrane space region with little negative charge.

Immunogen

membrane fraction from Vero (monkey kidney-derived) cells.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

  • Western blotting

Biochem/physiol Actions

Translocase of outer mitochondrial membrane 22 (TOMM22) is a key constituent of TOM complex. It functions as a mitochondrial receptor for the proapoptotic protein Bax (BCL2-associated X protein). TOMM22 interacts with 3-β hydroxysteroid dehydrogenase 2 (3βHSD2) and converts pregnenolone-to-progesterone in steroidogenic cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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An outer mitochondrial translocase, Tom22, is crucial for inner mitochondrial steroidogenic regulation in adrenal and gonadal tissues.
Rajapaksha M, et al.
Molecular and Cellular Biology, 36(6), 1032-1047 (2016)
Andrey Aristov et al.
Nature communications, 9(1), 2409-2409 (2018-06-21)
Single molecule localization microscopy can generate 3D super-resolution images without scanning by leveraging the axial variations of normal or engineered point spread functions (PSF). Successful implementation of these approaches for extended axial ranges remains, however, challenging. We present Zernike Optimized Localization
TOM22, a core component of the mitochondria outer membrane protein translocation pore, is a mitochondrial receptor for the proapoptotic protein Bax.
Bellot G, et al.
Cell Death and Differentiation, 14(4), 785-785 (2007)
Wei Ouyang et al.
Nature biotechnology, 36(5), 460-468 (2018-04-17)
The speed of super-resolution microscopy methods based on single-molecule localization, for example, PALM and STORM, is limited by the need to record many thousands of frames with a small number of observed molecules in each. Here, we present ANNA-PALM, a
Kei Okatsu et al.
The Journal of biological chemistry, 288(51), 36372-36384 (2013-11-06)
Parkinsonism typified by sporadic Parkinson disease is a prevalent neurodegenerative disease. Mutations in PINK1 (PTEN-induced putative kinase 1), a mitochondrial Ser/Thr protein kinase, or PARKIN, a ubiquitin-protein ligase, cause familial parkinsonism. The accumulation and autophosphorylation of PINK1 on damaged mitochondria
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