861251

Neutral Red

certified by the Biological Stain Commission

• Synonym(s): 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red
• Empirical Formula (Hill Notation): C₁₅H₁₇ClN₄
• CAS Number: 553-24-2
• Molecular Weight: 288.78
• Colour Index Number: 50040
• Beilstein: 3918740
• EC Number: 209-035-8
• MDL number: MFCD00012651
• UNSPSC Code: 12171500
• PubChem Substance ID: 24888533
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 100
• form: powder
• composition: Dye content, 50-60%
• technique(s): titration: suitable
• pH: 6.8-8.0, red to yellow
• mp: 290 °C (dec.) (lit.)
• solubility: water: 1 mg/mL
• λmax: 540 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1
• InChI: 1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
• InChI key: PGSADBUBUOPOJS-UHFFFAOYSA-N

Description

Application

Neutral red has been used as a staining agent for micro- and meso-plankton. It has been used as a staining agent for nematocytes.

Useful as an indicator for preparing neutral red paper, and as a biological stain.

Biochem/physiol Actions

Neutral red is useful as an indicator for preparing neutral red paper, and as a biological stain. Neutral dye has low toxicity and is a vital dye. It can also be used as a pH indicator. It is used for cell viability assay. The dye accumulates in the lysosomes of living healthy cells, but not in damaged cells.

Suitability

Certified for use in the method of Doan & Ralph for the supravital staining of living blood cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves