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Key Documents

IHCR1002-6

Sigma-Aldrich

Anti-APP A4 Antibody, a.a. 66-81 (NT), Prediluted, clone 22C11

clone 22C11, Chemicon®, from mouse

Synonym(s):

APP

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody product type

primary antibodies

clone

22C11, monoclonal

species reactivity

canine, mouse, monkey, pig, fish, human, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... APP(351)

General description

Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humand and cases of Alzheimer′s Disease. The principle component of this extracellular amyloid is beta/A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins are currently under investigation Reacts with pre-A4.

Specificity

The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000). The antibody is known to cross react with APLP2 (Slunt, 1994).

Immunogen

Epitope: a.a. 66-81 (N-terminus), Prediluted
Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.

Application

Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 30 minutes with IHC Select Detection Kits.

App has been prediluted for use as the primary antibody with Chemicon′s IHC Select Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier′s IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No. IHCR1002-6.
Detect Alzheimer Precursor Protein A4 using this Anti-APP A4 Antibody, a.a. 66-81 (N-terminus), Prediluted, clone 22C11 validated for use in IH(P).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Physical form

Format: Purified
Liquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as preservative.

Storage and Stability

Maintain at 2-8°C. Refer to vial for expiration dating.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Edward Rockenstein et al.
The Journal of biological chemistry, 280(38), 32957-32967 (2005-07-20)
Amyloid-beta peptides (Abeta) are widely presumed to play a causal role in Alzheimer disease. Release of Abeta from the amyloid precursor protein (APP) requires proteolysis by the beta-site APP-cleaving enzyme (BACE1). Although increased BACE1 activity in Alzheimer disease brains and

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