BI0166

Activator Reagent 0.3M BMT in AcN

Biosynthesis Novabiochem^®

• UNSPSC Code: 41116006

Properties

• Quality Level: 200
• product line: Novabiochem^®
• form: liquid
• reaction suitability: reaction type: Coupling Reactions; reagent type: catalyst
• transition temp: flash point 2 °C (Acetonitrile)
• shipped in: ambient
• storage temp.: 2-30°C

Description

General description

Activator Reagent 0.3M 5-benzylmercaptotetrazole (BMT) in acetonitrile (AcN) is also known as benzylthiotetrazole (BTT) or benzyl-1H-tetrazole). It is used as a phosphoramidite activator reagent for oligonucleotide synthesis.

Application

0.3M BMT in AcN can be used as a reagent in solid phase phosphorylation of the oligomers. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the 5′hydroxyl group of the growing oligonucleotide chain. The high activation potential of this reagent may be due to its greater acidity (pKa=4.08), which makes it easier to protonate the trivalent phosphorus in the initial stage of activation and increase the speed of the coupling reaction.

Features and Benefits

• efficient and rapid activation reagent that produces less
• phosphoramidite waste      
• higher solubility at lower temperatures      
• does not crystallize or clog lines       
• safer reagent to use

Analysis Note

Color (APHA): 10APHA max
Component: Acetonitrile Purity (By GC): 99.9% min
Form: Clear Liquid
Identity (IR-spectrum): Conforms
Molarity of 5-Benzylmercaptotetrazole: 0.28-0.32M
Water (H2O): 1-30ppm

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H302 + H312 + H332,H319
• Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 35.6 °F - closed cup - (Acetonitrile)
• Flash Point(C): 2 °C - closed cup - (Acetonitrile)