UHPLC Analysis of Diazepam and Metabolites on Titan C18, Competitive Comparison
CONDITIONS
column
Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)
mobile phase
[A] 0.1% formic acid; [B] acetonitrile; (60:40, A:B)
pressure
6450 psi (445 bar)
column temp.
35 °C
detector
UV, 254 nm
Descripción
Analysis Note
This application shows a comparison between Titan C18 and a competitive column for diazepam and its metabolites using a MS compatible mobile phase.
Other Notes
Used 75 μm I.D. x 250 mm L tubing in system.
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