HPLC Analysis of t-BOC-Phenylalanine Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] 20 mM ammonium acetate, pH 6; [B] methanol; (90:10, A:B)

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 220 nm

injection

1 μL

sample

1 mg/mL in methanol