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HPLC Analysis of Sugars on Ascentis® Si

HPLC Analysis of Sugars on Ascentis® Si application for HPLC

Materiales

analytical column

Referencia del producto
Descripción
Precios

581512-U

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Referencia del producto
Descripción
Precios

M8378

Maltotriosa

≥90% (HPLC)

84097

Sacarosa

BioUltra, for molecular biology, ≥99.5% (HPLC)

47287-U

Lactose Monohydrate

analytical standard

G0350500

D-(+)-Glucosa

European Pharmacopoeia (EP) Reference Standard

X0200000

Xylose

European Pharmacopoeia (EP) Reference Standard

1375025

Maltose monohydrate

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] water; [B] acetonitrile; (15:85, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

Descripción

Analysis Note

This application demonstrates the suitability of the Ascentis Si for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Si are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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