Saltar al contenido
MilliporeSigma

HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate

HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate application for HPLC

Materiales

Analytical column

Referencia del producto
Descripción
Precios

Z777033

Zenix® SEC-300 Gel Filtration Column, 3 μm

L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steel

mobile phase component

Referencia del producto
Descripción
Precios

452289

Ácido fosfórico

≥85 wt. % in H2O, ≥99.999% trace metals basis

standard

Referencia del producto
Descripción
Precios

M1882

Myoglobin from equine heart

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

U0750

Uracil

≥99.0%

T1001

Thyroglobulin from bovine thyroid

powder, ≥90% (agarose gel electrophoresis)

I4131

IgG from rat serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

05470

Seroalbúmina bovina

lyophilized powder, crystallized, ≥98.0% (GE)

CONDITIONS

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm (Z777033)

mobile phase

0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid

flow rate

0.1 mL/min

pressure

173 psi (12 bar)

column temp.

25 °C

detector

UV, 215 nm

injection

4 μL

sample

protein standards

Descripción

Analysis Note

Due to the low diffusion coefficents of proteins, optimal flow in size exclusion chromatography is often very low. However, the disadvantage is long run times. This example demonstrates both features.

Legal Information

Zenix is a registered trademark of Sepax Technologies

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS