HPLC Analysis of Phensuximide Enantiomers on Astec^® CYCLOBOND I 2000 DMP

column

CYCLOBOND I 2000 DMP, 25 cm x 4.6 mm I.D., 5 μm particles (20724AST)

mobile phase

[A] isopropanol; [B] hexane; (80:20, A:B)

flow rate

1 mL/min

column temp.

22 °C

detector

UV, 254 nm

injection

10 μL

sample

4 mg/mL in methanol