HPLC Analysis of Ketoconazole Enantiomers on Astec^® CYCLOBOND I 2000 HP-RSP

column

CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)

mobile phase

[A] 0.1% formic acid; [B] acetonitrile; (70:30, A:B)

flow rate

1 mL/min

column temp.

25 °C

injection

1 μL

detector

UV, 230 nm

sample

1 mg/mL in methanol