HPLC Analysis of Hemoglobin S on Discovery® BIO PolyMA-SCX, Ion Exchange
CONDITIONS
column
Discovery BIO PolyMA-SCX, 5cm × 4.6mm, 5μm particles (59601-U)
mobile phase
[A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0
gradient
0 to 100% B in 30 min. (linear)
flow rate
0.5 mL/min
column temp.
25 °C
detector
UV, 280 nm
injection
10 μL
Descripción
Analysis Note
Discovery BIO PolyMA-SCX and PolyMA-WAX columns give sharp, efficient peaks for a wide variety of proteins.
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany