Antihypertensive drugs (β-calcium channel blockers and ACE inhibitor)

column

Discovery RP-AmideC16, 15 cm × 4.6 mm I.D., 5 μm particles (505013)

mobile phase

[A] methanol; [B] 25 mM KH₂PO₄, pH 7.0 (40:60, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 214 nm

injection

5 μL