HPLC Analysis of Antibacterials/Antifungals on Ascentis^® C18

column

Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)

mobile phase

[A] 10 mM ammonium formate (pH 3.0 with formicacid); [B] acetonitrile; (75:25, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

5 μL

sample

50 μg/mL each in acetonitrile