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MilliporeSigma

Aroma volatiles from stored apples

Aroma volatiles from stored apples suitable for GC, application for SPME

Materiales

analytical column

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Precios

SLB®-5ms Capillary GC Column

L × I.D. 30 m × 0.25 mm, df 0.50 μm

SPME fiber

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SPME fiber assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB)

df 65 μm(PDMS/DVB, needle size 24 ga, for use with manual holder

related product

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Portafibra SPME

for use with manual sampling

Portafibra SPME

for use with CTC CombiPAL and Gerstel MPS2

standard

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Hexyl acetate

analytical standard

Butyl acetate

analytical standard

Isoamyl acetate

natural, ≥97%, FCC, FG

2-Metil-1-butanol

≥99%

Hexyl 2-methylbutanoate

analytical standard

Hexyl hexanoate

analytical standard

CONDITIONS

sample/matrix

300-450g intact fruit in a 3 liter flask at ambient temp, exposed to an air flow of 25-30 mL/min

SPME fiber

polydimethylsiloxane, 100 μm film (57310-U)

desorption process

250°C for 90 sec

column

5% phenyl polysiloxane, 25m × 0.25 mm I.D., 0.34 μm film (Supelco equivalent, SLB-5ms, 28473-U)

oven

40 °C (1.5 min) to 250 °C at 50 °C/min, hold 1 min

inj. temp.

250 °C

detector

mass spectrometer, m/z = 40-300 (40 spectra/sec)

carrier gas

helium, 1.5 mL/min (44 cm/min)

sample

Apples

Descripción

Analysis Note

SPME can reduce sampling time and is complementary to rapid separation/detection techniques such as time compressed gas chromatography (TCGC) and time-of-flight mass spectrometry (TOF-MS), to permit analysis of dozens of compounds in a few minutes. Investigators have used SPME to monitor flavor volatiles in apple, strawberry, tomato, and many other fruits and vegetables. In this application, 65 μm PDMS/divinylbenzene (DVB) SPME fibers were used to capture volatiles emanating from Mutsu apples stored for 3-4 months at 0 °C.