Saltar al contenido
MilliporeSigma
  • Preparation and evaluation of charged solid lipid nanoparticles of tetrandrine for ocular drug delivery system: pharmacokinetics, cytotoxicity and cellular uptake studies.

Preparation and evaluation of charged solid lipid nanoparticles of tetrandrine for ocular drug delivery system: pharmacokinetics, cytotoxicity and cellular uptake studies.

Drug development and industrial pharmacy (2013-05-15)
Jiawei Li, Xiujun Guo, Zhidong Liu, Chukwunweike Ikechukwu Okeke, Nan Li, Hainan Zhao, Mike Okweesi Aggrey, Weisan Pan, Tao Wu
RESUMEN

In this study, tetrandrine-loaded cationic solid lipid nanoparticles (TET-CNP) and solid lipid nanoparticles (TET-NP) were prepared by the emulsion evaporation-solidification at low temperature method. The particle size, zeta potential, and entrapment efficiency of TET-CNP and TET-NP were characterized. The results showed that the TET-CNP and TET-NP had average diameters of (15.29 ± 1.34) nm and (18.77 ± 1.23) nm with zeta potentials of (5.11 ± 1.03) mV and (-8.71 ± -1.23) mV and entrapment efficiencies of (94.1 ± 2.37)% and (95.6 ± 2.43)%, respectively. In vitro release studies indicated that the TET-CNP and TET-NP retained the drug entity better than tetrandrine ophthalmic solutions (TET-SOL). In the pharmacokinetics studies, the AUC values of TET-CNP and TET-NP were 1.96-fold and 2.00-fold higher than that of TET-SOL ( p < 0.05); the Cmax values of TET-CNP and TET-NP were 2.45-fold and 2.53-fold higher than that of the TET-SOL (p < 0.05), respectively. Cytotoxicity study showed that TET-CNP and TET-NP had no significant toxicity at low concentrations. Flow cytometry studies and confocal microscopy analysis demonstrated that calcein labeled NP (CA-NP) uptake by SRA 01/04 cells was much higher than those of calcein labeled CNP (CA-CNP) and calcein solution (CA-SOL).

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Calcein, Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Octadecylamine, ≥99% (GC)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Octadecylamine, ≥99.0% (GC)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Octadecylamine, technical grade, 90%
En este momento no podemos mostrarle ni los precios ni la disponibilidad