Saltar al contenido
MilliporeSigma

Genomic analysis of ADAR1 binding and its involvement in multiple RNA processing pathways.

Nature communications (2015-03-10)
Jae Hoon Bahn, Jaegyoon Ahn, Xianzhi Lin, Qing Zhang, Jae-Hyung Lee, Mete Civelek, Xinshu Xiao
RESUMEN

Adenosine deaminases acting on RNA (ADARs) are the primary factors underlying adenosine to inosine (A-to-I) editing in metazoans. Here we report the first global study of ADAR1-RNA interaction in human cells using CLIP-seq. A large number of CLIP sites are observed in Alu repeats, consistent with ADAR1's function in RNA editing. Surprisingly, thousands of other CLIP sites are located in non-Alu regions, revealing functional and biophysical targets of ADAR1 in the regulation of alternative 3' UTR usage and miRNA biogenesis. We observe that binding of ADAR1 to 3' UTRs precludes binding by other factors, causing 3' UTR lengthening. Similarly, ADAR1 interacts with DROSHA and DGCR8 in the nucleus and possibly out-competes DGCR8 in primary miRNA binding, which enhances mature miRNA expression. These functions are dependent on ADAR1's editing activity, at least for a subset of targets. Our study unfolds a broad landscape of the functional roles of ADAR1.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Magnesium chloride solution, for molecular biology, 1.00 M±0.01 M
Sigma-Aldrich
Magnesium chloride, anhydrous, ≥98%
Sigma-Aldrich
L-Glutamina, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source
Sigma-Aldrich
L-Glutamina
Sigma-Aldrich
BIS-TRIS, ≥98.0% (titration)
SAFC
L-Glutamina
SAFC
BIS-TRIS
Sigma-Aldrich
Magnesium chloride, powder, <200 μm
Sigma-Aldrich
Magnesium chloride solution, BioUltra, for molecular biology, 2 M in H2O
Sigma-Aldrich
Magnesium chloride, BioReagent, suitable for insect cell culture, ≥97.0%
Sigma-Aldrich
Ethyl acetoacetate, ReagentPlus®, 99%
Sigma-Aldrich
BIS-TRIS, BioXtra, ≥98.0% (titration)
Sigma-Aldrich
BIS-TRIS, BioUltra, ≥99.0% (NT)
Sigma-Aldrich
L-Glutamina, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
BIS-TRIS, BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%
Sigma-Aldrich
Magnesium chloride solution, BioUltra, for molecular biology, ~1 M in H2O
Sigma-Aldrich
Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR
Sigma-Aldrich
Ethyl acetoacetate, natural, ≥97%, FG
Sigma-Aldrich
Magnesium chloride, AnhydroBeads, −10 mesh, 99.9% trace metals basis
Sigma-Aldrich
L-Thyroxine sodium salt pentahydrate, γ-irradiated, powder, BioXtra, suitable for cell culture
USP
Levotiroxina, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
L-Glutamina, γ-irradiated, BioXtra, suitable for cell culture
Sigma-Aldrich
L-Glutamina
Sigma-Aldrich
L-Thyroxine sodium salt pentahydrate, ≥98% (HPLC), powder
SAFC
BIS-TRIS
Sigma-Aldrich
Magnesium chloride, AnhydroBeads, −10 mesh, 99.99% trace metals basis
Sigma-Aldrich
Magnesium chloride solution, 0.1 M
Sigma-Aldrich
Ethyl acetoacetate, puriss. p.a., ≥99.0% (GC)
Sigma-Aldrich
Ethyl acetoacetate, ≥99%, FCC, FG
Supelco
L-Glutamina, Pharmaceutical Secondary Standard; Certified Reference Material