Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.

Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.

Bio-medical materials and engineering (2014-09-18)

Wenfang Zhao, Kehai Liu, Shunsheng Chen, Man Man Zhu, Hui Lv, Jing Hu, Yuan Mao

RESUMEN

To solve the contradiction between the cell toxicity and transfection efficiency of polyethylenimine (PEI) derivate in non-viral gene therapy, a novel gene vector, P123-PEI-R18 was synthesized by using biodegradable PEI derivate conjugated with trifunctional peptide RGD-TAT-NLS. The particle size of P123-PEI-R18/DNA was around 100-250 nm. The gene vector could condense DNA at the weight ratio of 2 and protect plasmid DNA from being dissolved in the blood circulation. Importantly, the complexes exhibited lower cell toxicity and higher transfection efficiency contrasted with PEI 25 kDa in vitro. P123-PEI-R18 holds high potential as a safe and efficient gene vector.

MATERIALES

Referencia del producto

Marca

Descripción del producto

E7023

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34856

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Diclorometano, suitable for HPLC, ≥99.8%, contains amylene as stabilizer

459844

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319902

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D65100

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Diclorometano, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%

34858

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650463

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650528

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493546

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Alcohol etílico puro, 200 proof, meets USP testing specifications

270709

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Benceno, suitable for HPLC, ≥99.9%

E7148

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Alcohol etílico puro, 190 proof, for molecular biology

319953

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Benceno, ACS reagent, ≥99.0%

130672

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N-Hydroxysuccinimide, 98%

32222

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320269

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33211

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Acetato de etilo, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

439169

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Diclorometano, puriss., meets analytical specification of Ph. Eur., NF, ≥99% (GC)

676853

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Triethylamine, ≥99.5%

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Supelco

Diclorometano, Pharmaceutical Secondary Standard; Certified Reference Material