- A novel functional role for MMSET in RNA processing based on the link between the REIIBP isoform and its interaction with the SMN complex.
A novel functional role for MMSET in RNA processing based on the link between the REIIBP isoform and its interaction with the SMN complex.
PloS one (2014-06-14)
Fabio Mirabella, Alexander Murison, Lauren I Aronson, Christopher P Wardell, Andrew J Thompson, Sarah J Hanrahan, Jacqueline H L Fok, Charlotte Pawlyn, Martin F Kaiser, Brian A Walker, Faith E Davies, Gareth J Morgan
PMID24923560
RESUMEN
The chromosomal translocation t(4;14) deregulates MMSET (WHSC1/NSD2) expression and is a poor prognostic factor in multiple myeloma (MM). MMSET encodes two major protein isoforms. We have characterized the role of the shorter isoform (REIIBP) in myeloma cells and identified a clear and novel interaction of REIIBP with members of the SMN (survival of motor neuron) complex that directly affects the assembly of the spliceosomal ribonucleic particles. Using RNA-seq we show that REIIBP influences the RNA splicing pattern of the cell. This new discovery provides novel insights into the understanding of MM pathology, and potential new leads for therapeutic targeting.
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Cloruro de sodio, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
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Formaldehído solution, stabilized with methanol, ~37 wt. % in H2O, certified reference material
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Formaldehído solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
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Cloruro de sodio, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture
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Cloruro de sodio, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%