Saltar al contenido
MilliporeSigma

MUL1 acts in parallel to the PINK1/parkin pathway in regulating mitofusin and compensates for loss of PINK1/parkin.

eLife (2014-06-06)
Jina Yun, Rajat Puri, Huan Yang, Michael A Lizzio, Chunlai Wu, Zu-Hang Sheng, Ming Guo
RESUMEN

Parkinson's disease (PD) genes PINK1 and parkin act in a common pathway that regulates mitochondrial integrity and quality. Identifying new suppressors of the pathway is important for finding new therapeutic strategies. In this study, we show that MUL1 suppresses PINK1 or parkin mutant phenotypes in Drosophila. The suppression is achieved through the ubiquitin-dependent degradation of Mitofusin, which itself causes PINK1/parkin mutant-like toxicity when overexpressed. We further show that removing MUL1 in PINK1 or parkin loss-of-function mutant aggravates phenotypes caused by loss of either gene alone, leading to lethality in flies and degeneration in mouse cortical neurons. Together, these observations show that MUL1 acts in parallel to the PINK1/parkin pathway on a shared target mitofusin to maintain mitochondrial integrity. The MUL1 pathway compensates for loss of PINK1/parkin in both Drosophila and mammals and is a promising therapeutic target for PD.DOI: http://dx.doi.org/10.7554/eLife.01958.001.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Roche
Reactivo de transfección de ADN X-tremeGENE 9, Polymer reagent for transfecting common cell lines
Sigma-Aldrich
MISSION® esiRNA, targeting human MUL1