Saltar al contenido
MilliporeSigma

Transition states. Trapping a transition state in a computationally designed protein bottle.

Science (New York, N.Y.) (2015-02-24)
Aaron D Pearson, Jeremy H Mills, Yifan Song, Fariborz Nasertorabi, Gye Won Han, David Baker, Raymond C Stevens, Peter G Schultz
RESUMEN

The fleeting lifetimes of the transition states (TSs) of chemical reactions make determination of their three-dimensional structures by diffraction methods a challenge. Here, we used packing interactions within the core of a protein to stabilize the planar TS conformation for rotation around the central carbon-carbon bond of biphenyl so that it could be directly observed by x-ray crystallography. The computational protein design software Rosetta was used to design a pocket within threonyl-transfer RNA synthetase from the thermophile Pyrococcus abyssi that forms complementary van der Waals interactions with a planar biphenyl. This latter moiety was introduced biosynthetically as the side chain of the noncanonical amino acid p-biphenylalanine. Through iterative rounds of computational design and structural analysis, we identified a protein in which the side chain of p-biphenylalanine is trapped in the energetically disfavored, coplanar conformation of the TS of the bond rotation reaction.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
L-Alanine, ≥98% (TLC)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
L-Alanine, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
L-Alanine, BioUltra, ≥99.5% (NT)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
L-Alanine, ≥99%
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
Alanine, European Pharmacopoeia (EP) Reference Standard
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Supelco
L-Alanine, Pharmaceutical Secondary Standard; Certified Reference Material
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
L-Alanine-12C3, 99.9 atom % 12C
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Supelco
L-Alanine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
En este momento no podemos mostrarle ni los precios ni la disponibilidad