The biocompatibility of silver-containing Na2O.CaO.2SiO2 glass prepared by sol-gel method: in vitro studies.

This study was performed to determine the in vitro biocompatibility of a bioactive, antibacterial silver based gel. A human osteoblast-like cell line (HOS TE85) was used to test cytocompatibility in the presence of eluants and the biological response in direct contact with Na(2)O.CaO.2SiO(2) gel (containing 0.5050 wt % Ag(2)O prepared by a sol-gel process) sterilized by two different methods; autoclave and gamma-irradiation. MTT assay was used for the indirect evaluation of eluant toxicity and alamar Blue was performed to assess the biological response, which included cell viability and proliferation, and the results showed that these materials were cytocompatible.