Investigation of the silylation of ephedrines using N-methyl-N-trimethylsilyl-trifluoroacetamide.

Investigation of the silylation of ephedrines using N-methyl-N-trimethylsilyl-trifluoroacetamide.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2004-11-04)

Guro Forsdahl, Günter Gmeiner

RESUMEN

This paper describes a gas chromatography (GC)--mass spectrometry (MS) method for the simultaneous quantification of ephedrine, pseudoephedrine, cathine, norephedrine and methylephedrine in urine. The sample preparation step includes solid phase extraction and derivatisation with N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA). An evaluation of various silylation conditions compatible with screening methods in doping control analysis is presented. The method was found to be well suited for quantification of ephedrines in doping control.

MATERIALES

Referencia del producto

Marca

Descripción del producto

394866

Supelco

N-Metil-N-(trimetilsilil)trifluoroacetamida, synthesis grade

M7891

Supelco

N-Metil-N-(trimetilsilil)trifluoroacetamida, BioReagent, for silylations, LiChropur^™

69479

Supelco

N-Metil-N-(trimetilsilil)trifluoroacetamida, for GC derivatization, LiChropur^™, ≥98.5%

M-132

Supelco

N-Metil-N-(trimetilsilil)trifluoroacetamida, ampule of 10 × 1.2 mL, analytical standard, Cerilliant^®

69478

Supelco

N-Methyl-N-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane, for GC derivatization, LiChropur^™