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Establishment of a monitoring system to detect inhibition of mRNA processing.

Bioscience, biotechnology, and biochemistry (2012-07-14)
Ken-ichi Fujita, Masumi Okamura, Sachiko Nishimoto, Tomoya Kurihara, Keiko Momma, Yusaku Miyamae, Taiho Kambe, Masaya Nagao, Hiroshi Narita, Seiji Masuda
RESUMEN

A number of proteins complete mRNA processing in the nucleus, thus, inhibitor of mRNA processing is worth finding to analyze the mechanism of mRNA maturation in detail. Here, we established a monitoring system for mRNA processing using a test compound, spliceostatin A (SSA), which inhibits mRNA splicing. This system should serve to facilitate the discovery of novel compounds from natural resources that inhibit mRNA processing.

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TG003, ≥98% (HPLC)