- Over-expression of chloroperoxidase in Caldariomyces fumago.
Over-expression of chloroperoxidase in Caldariomyces fumago.
Biotechnology letters (2011-07-08)
Markus Buchhaupt, Kristina Ehrich, Sonja Hüttmann, Jan Guder, Jens Schrader
PMID21735258
RESUMEN
The filamentous fungus Caldariomyces fumago secretes a chloroperoxidase (CPO). To increase its production, we integrated a CPO-expression cassette into the non-transcribed spacer regions of the rDNA in C. fumago. One strain was obtained that had twice the CPO activity when grown in shake-flask and bioreactor compared to the wild-type. The highest CPO activity from the bioreactor cultivation was 3,236 U ml(-1). This is the highest value reported so far.
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Sigma-Aldrich
Chloroperoxidase from Caldariomyces fumago, buffered aqueous suspension, ≥3,000 units/mL
Sigma-Aldrich
Chloroperoxidase from Caldariomyces fumago, aqueous suspension, brown, >10,000 U/mL
Sigma-Aldrich
Chloroperoxidase from Caldariomyces fumago, buffered aqueous suspension, 1,000-2,000 units/mg protein (E1%/280)