Lung Section Staining and Microscopy.

Bio-protocol (2017-11-25)

Xiaofeng Zhou, Bethany B Moore

RESUMEN

Our protocol describes immunofluorescent staining, hematoxylin and eosin staining and Masson's trichrome staining on lung sections.

MATERIALES

Referencia del producto

Marca

Descripción del producto

Sigma-Aldrich

Ácido acético, glacial, ReagentPlus^®, ≥99%

Sigma-Aldrich

Ácido clorhídrico, ACS reagent, 37%

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Cloruro de hierro (III), reagent grade, 97%

Sigma-Aldrich

Ácido cítrico, ACS reagent, ≥99.5%

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Aluminum potassium sulfate dodecahydrate, ACS reagent, ≥98%

Sigma-Aldrich

Hematoxylin, certified by the Biological Stain Commission

Sigma-Aldrich

Eosin Y, Dye content ~99 %

Sigma-Aldrich

Sodium phosphate monobasic, BioXtra, ≥99.0%