- D-Ornithine coopts pyrrolysine biosynthesis to make and insert pyrroline-carboxy-lysine.
D-Ornithine coopts pyrrolysine biosynthesis to make and insert pyrroline-carboxy-lysine.
Nature chemical biology (2011-04-29)
Susan E Cellitti, Weijia Ou, Hsien-Po Chiu, Jan Grünewald, David H Jones, Xueshi Hao, Qian Fan, Lisa L Quinn, Kenneth Ng, Andrew T Anfora, Scott A Lesley, Tetsuo Uno, Ansgar Brock, Bernhard H Geierstanger
PMID21525873
RESUMEN
D-ornithine has previously been suggested to enhance the expression of pyrrolysine-containing proteins. We unexpectedly discovered that uptake of D-ornithine results in the insertion of a new amino acid, pyrroline-carboxy-lysine (Pcl) instead of the anticipated pyrrolysine (Pyl). Our feeding and biochemical studies point to specific roles of the poorly understood Pyl biosynthetic enzymes PylC and PylD in converting L-lysine and D-ornithine to Pcl and confirm intermediates in the biosynthesis of Pyl.
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