Saltar al contenido
MilliporeSigma

Toxicity of the biocide polycarbamate, used for aquaculture nets, to some marine fish species.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP (2018-09-12)
Kazuhiko Mochida, Katsutoshi Ito, Mana Ito, Takeshi Hano, Nobuyuki Ohkubo
RESUMEN

We investigated toxic effects of the antifouling biocide polycarbamate (PC) on marine fish by conducting acute, early-life stage toxicity (ELS), and embryo toxicity tests. Mummichog (Fundulus heteroclitus) 96-h LC50 values for hatched larvae (body weight about 2.0 mg) and juveniles (660 ± 36 mg) were about 12 and 630 μg/L, respectively. The ELS test using mummichog embryos yielded a lowest-observed-effect concentration of 3.9 μg/L and a no-observed-effect concentration of 2.1 μg/L with growth as the most sensitive endpoint. The embryo toxicity test for spotted halibut (Verasper variegatus) revealed a 10-d EC50 of 8.1 μg/L with abnormality as an endpoint. During the ELS and embryo toxicity tests, morphological abnormalities (notochord undulation) were induced in the embryos. Biochemical and gene-expression analysis suggest that PC-induced morphological abnormalities involve disruption of lysyl oxidase-mediated collagen fiber organization, essential for notochord formation, and inhibition of gene expression related to notochord formation.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Ampliflu Red, for fluorescence, ≥98.0% (HPLC)
Sigma-Aldrich
2-Mercaptopyridine N-oxide, 99%