Non-Fat Dry Milk (NFDM) Solution for Blocking Western Blots

Non-fat dry milk (NFDM) blocking buffer is ideal for saturating excess protein-binding sites on membranes for Western blotting. The primary proteins in milk are casein and whey. Typically, 1-5% NFDM is sufficient for most Western blots. To make a 1% solution of NFDM, combine 1 g of NFDM and 100 mL of TBST.

This calculator enables the accurate NFDM solution preparation to make enough for a single experiment or for the entire lab. Input your desired concentration and volume, click the CALCULATE button, and the table will populate with the amounts of each component needed.

Non-fat Dry Milk Buffer Recipe Calculator

OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS

Stock Solutions

• 4X SDS Sample Loading Buffer
• 10X Phosphate-Buffered Saline (PBS)
• 10X Tris-Buffered Saline (TBS) Stock Solution
• Stock Components for Western Blot Stripping Solutions

Wash & Blocking Buffers

• 1X Tris-Buffered Saline (TBS) Working Solution
• 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
• 1X PBS (Phosphate-Buffered Saline)
• 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
• Bovine Serum Albumin (BSA) Blocking Buffer

Stripping Solutions

• Detergent-based Western Blot Stripping Buffer
• Acidic Western Blot Stripping Solution
• Stock Components for Western Blot Stripping Solutions