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MilliporeSigma

SEP080

Millipore

Seppro® Rubisco

LC2

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

column D × L

6.4 mm × 63 mm

capacity

1 mg total protein loading(D-Ribulose 1,5-Diphosphate Carboxylase (Rubisco))

storage temp.

2-8°C

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General description

RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase) is the most abundant protein in plants, and it may be the most abundant protein on Earth. RuBisCO is an enzyme that is used in the Calvin cycle to catalyze the first major step of carbon fixation, a process by which the atoms of atmospheric carbon dioxide are made available to organisms in the form of energy-rich molecules such as sucrose. RuBisCO, while being the key enzyme in photosynthetic carbon assimilation in green leaves, is the main obstacle in plant proteomics. It constitutes about 40% of the total protein mass in green leaves, thus interfering with proteomics studies such as LC-MS/MS and 2D-gel electrophoresis. To meet the needs for specifically and effectively separating RuBisCO from other plant proteins, Sigma developed a novel immunoaffinity matrix based on the IgY antibodies cross-linked to microbeads, which specifically removes RuBisCO protein from plant extract.

Application

Seppro® Rubisco, LC2 (2mL columns) may be used in plant proteomics research to effect IgY-mediated immune affinity removal of RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase) from plant tissue extracts via liquid chromatography.

Legal Information

Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • S4199Seppro® Dilution bufferSDS

  • S4324Seppro® Stripping BufferSDS

  • S4449Seppro® Neutralization BufferSDS

  • CLS8163Corning® Costar® Spin-X® centrifuge tube filters, cellulose acetate membrane, pore size 0.45 μm, non-sterileSDS

Storage Class

10 - Combustible liquids


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Renato Millioni et al.
PloS one, 6(5), e19603-e19603 (2011-05-17)
To date, the complexity of the plasma proteome exceeds the analytical capacity of conventional approaches to isolate lower abundance proteins that may prove to be informative biomarkers. Only complex multistep separation strategies have been able to detect a substantial number

Questions

1–5 of 5 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. Can I do TCA/acetone precipitation for isolating plant proteins to use with the Seppro® Rubisco kit, Product SEP080?

    1 answer
    1. TCA/acetone precipitation is problematic because it may be difficult to redissolve the protein pellet afterwards. The following paper provides some tips for extraction: Cellar, N.A., et al., J. Chromatography B, 861, 29-39 (2008).

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  3. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  4. How can I extract my plant proteins for use with Seppro® Rubisco kit, Product SEP080?

    1 answer
    1. One suggestion is to use PBS with 0.05% Tween 20. Extraction with urea/SDS is not suggested because this is incompatible with the antibodies in the Seppro products.

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  5. I originally purchased my Seppro RuBisCO LC2 column from Genway. Can I still contact Genway for technical support?

    1 answer
    1. All inquiries on the Seppro technology are now to be referred to Sigma-Aldrich. Customers should not contact Genway for technical assistance.

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