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S6639

Sigma-Aldrich

SSC Buffer 20× Concentrate

for Northern and Southern blotting, solution

Sinónimos:

20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

description

Saline-Sodium Citrate buffer, made with ultrapure water

sterility

0.2 μm filtered

form

solution

application(s)

diagnostic assay manufacturing

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

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General description

SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.

Application

SSC Buffer 20× Concentrate has been used
  • as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
  • as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
  • as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Components

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

related product

Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Chronic hypoxia facilitates Alzheimer's disease through demethylation of gamma-secretase by downregulating DNA methyltransferase 3b
Liu H, et al.
Alzheimers Dement., 12(2), 130-143 (2016)
L1 retrotransposon expression in circulating tumor cells
Papasotiriou I, et al.
Testing, 12(2), e0171466-e0171466 (2017)
Alexandros Sountoulidis et al.
PLoS biology, 18(11), e3000675-e3000675 (2020-11-21)
Changes in cell identities and positions underlie tissue development and disease progression. Although single-cell mRNA sequencing (scRNA-Seq) methods rapidly generate extensive lists of cell states, spatially resolved single-cell mapping presents a challenging task. We developed SCRINSHOT (Single-Cell Resolution IN Situ
Chang-Gong Liu et al.
Methods (San Diego, Calif.), 44(1), 22-30 (2007-12-26)
After 12 years from its first application, microarray technology has become the reference technique to monitor gene expression of thousands of genes in the same experiment. In the past few years an increasing amount of evidence showed the importance of
Michaela Asp et al.
Scientific reports, 7(1), 12941-12941 (2017-10-13)
Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In
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