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MilliporeSigma

R6504

Sigma-Aldrich

Medio RPMI-1640

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

El medio RPMI 1640 lo desarrolló el equipo de Moore en el Instituto Roswell Park Memorial en 1966. Se formuló una modificación del medio McCoy 5A para sostener las células linfoblastoides en cultivo en suspensión, pero desde entonces se ha demostrado que sirve de soporte para una amplia variedad de células que necesitan anclaje. Originalmente indicado para utilizar con un complemento sérico, se ha demostrado que el RPMI 1640 respalda diversas líneas celulares en ausencia de suero. También se ha utilizado ampliamente en protocolos de fusión y en el crecimiento de células híbridas. Este medio es adecuado para el cultivo de leucocitos humanos sanos y neoplásicos.
Roswell Park Memorial Institute medium is commonly known as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. It is used for the growth of human lymphoid cells.

Application

RPMI-1640 Medium is used for the following applications:
  • used in cell culture medium (i.e. Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components)
  • used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
  • used for cell isolation and culture
  • used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo proliferation assay
  • used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
  • used for the preparation of antifungal agents

Quantity

Formulated to contain 10.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Artículos

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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