SCM144
BrightCell MEMO Photostable Media
Live cell imaging cell culture media (DMEM), 500ml
About This Item
Productos recomendados
form
liquid
technique(s)
cell culture | stem cell: suitable
shipped in
ambient
General description
Protocol
Some of the phototoxic components present in standard media (but removed from MEMO/NEUMO) contribute to cellular proliferation. Consequently, MEMO/NEUMO should only be used during the phase of the experiment in which cells are exposed to prolonged periods of light. SOS, in contrast, supports cellular viability and proliferation equal to alternative (phototoxic) supplements and should be used continuously during cell maintenance.
SOS should be used to replace neuronal supplements, such as B-27, if you are currently using these in your media. If you are presently using FBS, this can be harmful to cells under intensive light. Replacement of FBS with SOS may help to support the growth of cells, especially when used in conjunction with cell line specific growth factors.
Media Preparation
1. Thaw SOS at 37°C. SOS is supplied as a 25X concentrate and should be added to MEMO/NEUMO to a final concentration of 4%. Any liquid remaining should be aliquoted into working volumes and store at -20°C.
Note: Avoid freeze-thawing of SOS supplement more than twice.
2. Add additional components in an aseptic manner according to Table 1 in the userguide.
3. Once supplemented, the complete media is stable for 2 weeks when stored at 4°C.
Cell Culture
1. Initially expand/maintain cells in the dark using Neurobasal supplemented with SOS.
2. The evening before exposure to prolonged periods of light, replace Neurobasal/SOS with pre-warmed MEMO/NEUMO and SOS. Cells will maintain viability for up to 3 days if required.
a.Aliquot media to pre-heat, repetitive heating and cooling of bottles of media may cause precipitation.
3. Perform experiment requiring exposure to light.
4. Once exposure to light has been completed, remove the MEMO/NEUMO and SOS and replace with Neurobasal/SOS.
BrightCell is a trademark of Merck KGaA, Darmstadt, Germany.
MEMO, NEUMO and SOS are registered trademarks of Cell Guidance Systems LLC.
Neurobasal and B-27 are registered trademarks of Life Technologies.
Surpassing light-induced cell damage in vitro with novel cell culture media. Nature Scientific Reports (Learn More!)
The BrightCell photostable media system is cell culture media and supplements that have been reformulated with specific phototoxic components eliminated or replaced. The products allow prolonged exposure of cells to blue light while maintaining high levels of cell viability and functionality. Furthermore, BrightCell media products have very low levels of auto-fluorescence and photo-bleaching, dramatically improving the quality of data that can be obtained using fluorescent live cell imaging.
Application
Cell Culture
Components
2) One bottle containing 5ml of Supplement A (SCM149). Store at -20°C.
Quality
Osmolality: 320-360 mOsm/kg H2O
Sterility: Negative
Functional Test: Pass
Storage and Stability
Disclaimer
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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