AB5054
Anti-Calretinin Antibody
serum, Chemicon®
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
serum
clon
polyclonal
reactividad de especies
human, rat
fabricante / nombre comercial
Chemicon®
técnicas
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
Información sobre el gen
human ... CALB2(794)
Descripción general
Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.
Especificidad
Reactivity with other species has not been determined
Specific for calretinin. Recognizes both the calcium-bound and calcium-unbound conformations of calretinin by western blots.
Inmunógeno
Recombinant rat calretinin.
Aplicación
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
This Anti-Calretinin Antibody is validated for use in IH, IH(P), WB for the detection of Calretinin.
Western blot:
1:1,000-1:2,000 of a previous lot was used.
Immunohistochemistry:
1:1,000-1:10,000
Antibody works in paraffin embedded tissues.
Optimal working dilutions must be determined by the end user.
1:1,000-1:2,000 of a previous lot was used.
Immunohistochemistry:
1:1,000-1:10,000
Antibody works in paraffin embedded tissues.
Optimal working dilutions must be determined by the end user.
Calidad
Immunohistochemistry(paraffin) Analysis:
Calretinin (cat. # AB5054) staining on Human Cerebellum. Tissue pretreated with Citrate, pH 6.0 plus. Antibody was diluted to 1:200 (5.0 µg/mL), using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as a nuclear and cytoplasmic punctate staining on neurons (brown).
Optimal Staining With Citrate Buffer Epitope Retrieval: Human Cerebellum.
Calretinin (cat. # AB5054) staining on Human Cerebellum. Tissue pretreated with Citrate, pH 6.0 plus. Antibody was diluted to 1:200 (5.0 µg/mL), using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as a nuclear and cytoplasmic punctate staining on neurons (brown).
Optimal Staining With Citrate Buffer Epitope Retrieval: Human Cerebellum.
Ligadura / enlace
Replaces: AB149
Forma física
Rabbit polyclonal serum contains no preservative.
Unpurified
Almacenamiento y estabilidad
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Nota de análisis
Control
Rat brain cortex, Mesothelioma tissue, Human cerebellum.
Rat brain cortex, Mesothelioma tissue, Human cerebellum.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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