16-452
4G10® Platinum, Anti-Phosphotyrosine Antibody, Biotin Conjugate
clone 4G10®, Upstate®, from mouse
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Seleccione un Tamaño
100 μL
$803.00
Seleccione un Tamaño
Cambiar Vistas
100 μL
$803.00
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
biotin conjugate
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
4G10®, monoclonal
reactividad de especies (predicha por homología)
all
fabricante / nombre comercial
Upstate®
técnicas
western blot: suitable
isotipo
IgG2bκ
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... PID1(55022)
Descripción general
The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. Now Millipore has made the best even better with 4G10 Platinum. We pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20, to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone is capable.
Inmunógeno
The immunogen for 4G10 was phospho-tyramine coupled to KLH, while the immunogen for PY20 was phosphotyrosine conjugated to a carrier protein.
Aplicación
4G10 Platinum, Anti-Phosphotyrosine, Biotin Conjugate detects levels of Phosphotyrosine proteins & has been published & validated for use in IP.
Calidad
Western Blot Analysis:
A dilution of 1:1000-2000 of this antibody detected tyrosine-phosphorylated proteins in a modified RIPA lysate from human HEK293 cells treated with 100 nM insulin.
A dilution of 1:1000-2000 of this antibody detected tyrosine-phosphorylated proteins in a modified RIPA lysate from human HEK293 cells treated with 100 nM insulin.
Descripción de destino
Varies
Forma física
100 μL of a proprietary mixture of biotin conjugated, protein G purified mouse monoclonals 4G10 (IgG2bκ) and PY20 (IgG2b) in PBS, pH 7.4 with 0.05% sodium azide.
Almacenamiento y estabilidad
Stable for 6 months at 2-8°C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
NOTE: DO NOT FREEZE.
NOTE: DO NOT FREEZE.
Información legal
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Ren Liu et al.
Blood, 116(2), 297-305 (2010-05-06)
Axl is an oncogenic receptor tyrosine kinase that plays multiple roles in tumorigenesis and metastasis of many cancers. This study is the first to demonstrate that Axl is induced in Kaposi sarcoma and Kaposi sarcoma herpesvirus (KSHV) transformed endothelial cells.
Avin Hawez et al.
Laboratory investigation; a journal of technical methods and pathology (2021-11-05)
Sepsis is associated with exaggerated neutrophil responses although mechanisms remain elusive. The aim of this study was to investigate the role of c-Abelson (c-Abl) kinase in neutrophil extracellular trap (NET) formation and inflammation in septic lung injury. Abdominal sepsis was
Shalini Chaturvedi et al.
International journal of biological sciences, 9(10), 1099-1107 (2013-12-18)
A novel assay was developed to measure ratio of p-FMS (phospho FMS) to FMS using the Meso Scale Discovery(®) (MSD) technology and compared to the routinely used, IP-Western based approach. The existing IP-Western assay used lysed PBMCs (Peripheral Blood Mononuclear
Kasper J Mygind et al.
International journal of molecular sciences, 25(11) (2024-06-19)
Desmoplasia is a common feature of aggressive cancers, driven by a complex interplay of protein production and degradation. Basigin is a type 1 integral membrane receptor secreted in exosomes or released by ectodomain shedding from the cell surface. Given that
Philippe Foubert et al.
The Journal of clinical investigation, 117(6), 1527-1537 (2007-05-19)
Endothelial progenitor cell (EPC) transplantation has beneficial effects for therapeutic neovascularization; however, only a small proportion of injected cells home to the lesion and incorporate into the neocapillaries. Consequently, this type of cell therapy requires substantial improvement to be of
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
