SEQR
SeqPlex RNA Amplification Kit
For use with high throughput sequencing technologies
Sinónimos:
WGA kit, Whole transcriptome amplification
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About This Item
UNSPSC Code:
41121800
NACRES:
NA.55
Productos recomendados
technique(s)
whole genome amplification: suitable
shipped in
wet ice
storage temp.
−20°C
Categorías relacionadas
General description
The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.
application
SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.
Features and Benefits
- Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
- Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
- No need to fragment DNA before sequencing.
- Amplifies ds-cDNA in 8 hours or less.
- Compatible with all next generation sequencing platforms except Pacific Bioscience.
Other Notes
SEQR-500RXN is manufactured on-demand. Contact us for more information.
Legal Information
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
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Referencia del producto
Descripción
Precios
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Protocolos
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
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