mAbs Upstream
Products for Development and Manufacturing.
The upstream workflow encompasses all steps leading to cell harvest including optimization of culture conditions, cell line and product characterization, media development, upstream process chemicals; and scale-up of the batch, fed-batch or perfusion process to larger bioreactors. The goal of upstream biomanufacturing is to increase cell density and product titer.
Bioreactors
Bioreactors are available in a range of sizes and formats (stainless steel, single-use, or hybrid) to support both adherent and suspension cell cultures. Adherent cell lines can be grown in batch or fed-batch modes, or with media exchange, while suspension cells can be grown in batch, fed-batch, and perfusion modes. Regardless of the cell type or operating mode, the bioreactor must enable consistent cell growth, high cell viability, and robust protein production. During process development, scaled-down, small working volume microbioreactors are used to predict the performance of larger scale vessels, facilitating the process of scale-up for clinical and commercial production or tech transfer.
Our comprehensive offering around the bioreactor includes:
- Millipore® Single-Use Bioreactors With or Without Cell Retention System for Perfusion Operations
- Millipore® Sterilizing Filters & Prefilters
- Millipore® Sampling Solutions (automated online system, offline sampling)
- Millipore® Process Analytical Technology (Raman Analyzer, automated aseptic sampling)
- Millipore® CTDMO, BioReliance® / Millipore® Services (cell and viral bank testing, raw material testing, bulk harvest release testing / single-use and filter validation, equipment installation, system qualification, system training, system services)
- Millipore® Automation and Analytics Software
Related Categories
A cell line that maintains favorable growth and productivity is vital for mab manufacturing.
Our cell culture media makes your intensified or fed-batch processes more robust and efficient.
Our recombinant, non-animal origin (NAO) supplements are formulated for cell culture applications.
Mobius® single-use mixing solutions include a complete range of mixers from 10 L to 3,000 L.
The Mobius® Bioreactor family accommodates batch, fed-batch, and perfusion upstream processes.
Our broad portfolio of membrane filters offers options for efficient processing of every pharma and biopharma stream.
Related Resources
- Data Sheet: Mobius Breez Microbioreactor
This document highlights the benefits of the Mobius® Breez Microbioreactor, a 2 mL automated single-use perfusion cell culture platform.
- Accelerate Perfusion Media Development and Predict Large-Scale Processes using a Single-use, Automated Microbioreactor
This page describes the use of the Mobius® Breez Microbioreactor to rapidly screen and optimize media formulations for perfusion processes and to predict the performance of larger scale bioreactors as an alternative to traditional small-scale models.
- Data Sheet: Mobius® 3L Bioreactor
This document highlights the benefits of the Mobius® 3L Bioreactor.
- Data Sheet: Mobius® iFlex Bioreactor
This document highlights the benefits of Mobius® iFlex Bioreactor, a scalable family of next generation high-performance single-use modular bioreactors for fed-batch and perfusion processes.
- Scalability and Performance of Mobius® iFlex Bioreactors
Mobius® iFlex Bioreactors are single-use, stirred tank systems designed to support traditional and intensified fed-batch as well as perfusion cell culture applications. Several performance attributes and scalability data for these bioreactors are described in this article.
- Optimizing Upstream Media and Feeds in Bioprocessing
Developing a robust upstream cell culture process that maximizes productivity without increasing complexity relies on the right combination of bioprocess medium and feed and the ability to maintain tight control of culture conditions. Learn more how to achieve this.
- Upstream Bioprocessing Feed Strategies and Scalability: A Case Study with Cellvento® ModiFeed Prime COMP
Prior to scaling your upstream bioprocess to bioreactors, it’s best to set up small-scale cultures to determine the media and feed combinations as well as other parameters to best optimize growth, productivity, and product quality from your cells.
- In-Line Real-Time Monitoring of CHO Cell Culture Process Parameters using Raman Spectroscopy
This article explains how ProCellics™ Raman Analyzer with Bio4C® PAT Raman Software (the Raman PAT Platform) can be used to perform in-line and real-time measurement of TCD, VCD and mAb titer as well as the concentration of glucose, lactate, and ammonium in a bench-scale bioreactor, and how it compares with off line monitoring.
- Brochure: Mission Control: Strategies for Effective Bioburden and Aseptic Control
In this series of articles, we provide information to highlight the differing objectives for bioburden control by unit operation and discuss the parameters that guide filter selection and optimization.
- Cell Culture Media Filtration, Filter Selection and Sizing
The purpose of this application note is to provide estimated filtration areas for different sterilizing-grade filters with a panel of media used for Chinese Hamster Ovary (CHO) cell culture processes.
- Cell Culture Media Mixing and Microfiltration: Reducing Risk with Single-use Technology
This case study presents our recommended methods for thoroughly mixing media, as well as reducing the risk of mycoplasma contamination through the use of 0.1 μm sterilizing-grade filters.
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