Here we present the first genome wide, arrayed guide RNA screening libraries for CRISPR-Cas9. These libraries have been designed to cover 17166 human and 20430 mouse genes. To test the efficiency, we selected gRNAs targeting genes from the GPI anchor protein pathway. We observed that over 95% of these gRNAs successfully induced DNA cleavage and consequently gene loss of function. These libraries offer the prospect for performing high-throughput screens for genes of considerable scientific and therapeutic value in mouse and human cells.
Speakers
Emmanouil Metzakopian, Ph.D.
Wellcome Trust Sanger Institute
WTSI Career Development Fellow PDUK Senior Research Fellow
Dr. Metzakopian is a research fellow at Wellcome Trust Sanger Institute, where he focuses on identifying genes which confer resistance to chemotherapy drugs in embryonic stem cells and to oxidative stress agents in dopamine neurons.
Shawn Shafer, Ph.D.
Advanced Genomics Market Segment Manager
MilliporeSigma
Dr. Shafer leads a team responsible for product development of MilliporeSigma’s RNAi and genome editing portfolios. He orchestrated the creation of MilliporeSigma CRISPR collection of reagents.
Genomics
- Advanced gene editing
Duración:1h 3min
Idioma:English
Sesión 1:presentado May 12, 2016
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