To respond rapidly to urgent customers inquiries for recommendations to increase AAV titers in established AAV production processes, we evaluated a selection of our company's media and feed products as supplements during AAV production. We found Cellvento® ModiFeed Prime significantly increased AAV titers in HEK293 cell lines when supplemented post-transfection and in Sf-9 cell lines when supplemented post-infection. The volume and timing of the supplementation are critical for maximizing AAV titers and will be reviewed during this webinar.
In this webinar, you will learn:
- How to boost AAV titers in HEK293 and Sf-9 cell lines
- Insider tips on volume and timing optimization
Speaker
Jessica J. Haywood
MilliporeSigma
R&D Scientist
Jessica Haywood is an R&D scientist on the VGT & vaccine media development team at MilliporeSigma. Jessica earned her bachelor of science in molecular biology from Baker University in 2005. She has worked at our company for 14 years, during which time she has applied her extensive cell culture experience with HEK293, Sf-9, and CHO cell lines to developing custom and catalog cell culture media. Recently, Jessica was the technical lead for Cellvento® 4HEK medium launched in 2022 for AAV production via HEK293 transient transfections.
Pharma and biopharma manufacturing
- Cell Line Development
Duración:1h
Idioma:English
Sesión 1:presentado June 18, 2024
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